Abstract
Often the aim of a transformation is to obtain at least one transformant that can be used in further experiments. This transformant should contain either a linear fragment integrated at the correct position in the genome or a plasmid. On the other hand, for transformation with a plasmid library, for example, high transformation efficiency is required. Therefore, the proce-dures for transformation of K. lactis described here are optimized to obtain as many transformants as possible.
Cite
CITATION STYLE
Kooistra, R. A., & Steensma, H. Y. (2003). Transformation of Kluyveromyces lactis. In Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology (pp. 169–174). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-55758-3_26
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