Morphological and Molecular Identification of Arbuscular Mycorrhizal (AM) Fungi

Abstract

Mycorrhiza is important fungi for agriculture. To study of that plant’s root and soil samples were collected from the different regions of junagadh and near to junagadh district. AMF spores were isolated by Wet-sieving-decanting method and Adhesion- Flotation Technique. Morphological studies followed by tryphan blue staining revealed different stages with different components of AM fungi in compound microscope. Different size of spores 10 um, 20 um, 53 um and 105 um were observed in Scanning Electron Microscope (SEM). DNA of AMF colonized root were extracted by CTAB method. Using universal primers (A) AML1 and AML2 (B) NS 1 and NS 4 have been used for the identification of AM fungi they generated a single clear band around 800 bp and 1100 bp. For identification of specific mycorrhizal species Glomus, Aculospora or Gigaspora specific primers ITS1F, ITS4R, GLOM1310F, GLOM5.8R, GIGA5.8R, ARCH1311F,ARCH1311F, ACAU1660F and LETC1670F used.