Sublethal and Hormesis Effects of Clothianidin on the Black Cutworm (Lepidoptera: Noctuidae)

Abstract

The black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), has been considered a major insect pest in China that causes damage to corn at the seeding stage. The present study measured the lethal and sublethal effects of the neonicotinoid insecticide clothianidin on A. ipsilon. Clothianidin, incorporated using an artificial diet, exhibited signs of active toxicity on fourth-instar larvae of A. ipsilon, with a 50%-lethal concentration (LC 50 ) of 27.77 μg/g. Clothianidin at the LC 20 and LC 40 levels impaired the normal development of A. ipsilon by prolonging the larval period, decreasing the rate of pupation and eclosion, reducing longevity, shortening the oviposition period, and reducing the fecundity of female adults. Consequently, these effects resulted in the reduction of some population parameter values of A. ipsilon, including the intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R 0 ), along with an increase in the mean generation time (T). However, stimulatory effects, i.e., hormesis, on reproduction were observed in A. ipsilon exposed to an LC 5 level based on the fecundity (2,213.62 eggs per female) and net reproductive rate (R 0 ) (863.04 offspring per individual), which were significantly higher than values in the control group (1,344.77 eggs per female and 591.82 offspring per individual). Therefore, the results obtained in this study may assist in the development of optimized integrated pest management strategies, although the results require further study for corroboration under real cropping conditions.