Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management

Abstract

Context: Salvadora persica L. (Salvadoraceae), Colophospermum mopane (J.Kirk ex Benth.) J. Léonard (Leguminosae) and Dichrostachys cinerea (L.) Wight & Arn. (Leguminosae) crude extracts are used by local farmers against many livestock infections with little or no side effects usually associated with synthetic antimicrobials. However, their efficacy has rarely been tested. Objective: These plants were tested for potential antibacterial activity against clinical isolates of Staphylococcus aureus ATCC33862 and Escherichia coli ATCC25922. Minimal inhibitory concentrations (MIC) of the crude plant extracts were determined. Materials and methods: Aqueous and methanol extraction of 100 g each of the bark of C. mopane, roots of D. cinerea and leaves of S. persica was done by placing the samples in 250mL of either water or methanol. Nutrient broth was used as growth medium for the bacteria, and McFarland standard for bacterial standardization. 2,3,5-Triphenyltetrazoliumchloride (TTC) was the indicator salt. Each of the aqueous and methanol extracts (100 µL) was tested. Gentamycin and ampicillin were the controls. Results: MIC of aqueous extracts ranged from 1.03-14.6mg/mL against S. aureus, and from 12.1-34.3 mg/ mL against E. coli. Methanol extracts ranged between 5.31 and 9.64 mg/mL against S. aureus, and between 7.86 and 13.6mg/mL against E. coli. Aqueous and methanol extracts of S. persica were significantly higher (p<0.05) than C. mopane and D. cinerea. Discussion and conclusion: Colophospermum mopane, S. persica and D. cinerea exhibited antibacterial activity, with methanol extracts performing better than aqueous extracts, justifying use as ethnoveterinary medicine. Further study to isolate the active components should be pursued.