Overexpression of Aiolos in Nalm-6 acute lymphoblastic leukaemia cells reduces apoptosis by suppressing phosphatase and tensin homologue deleted on chromosome 10 and activating the phosphatidylinositol-3-kinase/Akt signalling pathway

Abstract

The aim of the present study was to elucidate the molecular mechanism of Aiolos in the regulation of B-cell leukaemia. A lentiviral system was used for overexpression of the Aiolos gene in Nalm-6 cells to determine the effects of Aiolos on proliferation, apoptosis and the cell cycle. The expression and activation of phosphatase and tensin homolog deleted on chromosome ten (PTEN) and Akt were also investigated. Upregulation of Aiolos inhibited cell growth and arrested an increased number of Nalm-6 cells at the G0/G1 phase. The apoptotic cell quantities were also significantly lower in the Aiolos-transfected Nalm-6 cells. In addition, Aiolos overexpression downregulated PTEN, but increased the expression and phosphorylation of Akt in the Nalm-6 cells. The Akt inhibitor, Akti-1/2, reduced the percentage of viable Aiolos-overexpressed Nalm-6 cells, however, it had no effect on cell cycle arrest or proliferation. Aiolos upregulation in the Nalm-6 cells inhibited cell proliferation, suppressed apoptosis and arrested the cell cycle at the G0/G1 phase. Aiolos improved the survival of Nalm-6 cells via PTEN-and Akt-dependent processes.