Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine

72Citations
Citations of this article
24Readers
Mendeley users who have this article in their library.

Abstract

Mutations in three Escherichia coli K-12 genes were isolated that reduce the efficiency of the lysine-inserting nonsense suppressor supL. These antisuppressor mutations asuD, asuE, and asuF map at 61.9, 25.3, and 76.3 min, respectively, on the E. coli chromosome. Biochemical and genetic analysis of the mutant strains revealed the reason for the antisuppressor phenotype for two of these genes. The activity of lysyl-tRNA synthetase was reduced in strains with asuD mutations. The modification of 5-methylaminomethyl-2-thiouridine, the wobble base of tRNA(Lys), was impaired in asuE mutant strains, presumably at the 2-thiolation step.

Cite

CITATION STYLE

APA

Sullivan, M. A., Cannon, J. F., Webb, F. H., & Bock, R. M. (1985). Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine. Journal of Bacteriology, 161(1), 368–376. https://doi.org/10.1128/jb.161.1.368-376.1985

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free