Flow cytometry is a powerful tool for the analysis of apoptosis, the process that directly determines cell fate after the action of different stresses. Here, we describe a flow cytometry method for the assessment of early and late stages of apoptosis in non-fixed cultured cells using SYTO16, DRAQ7, and PO-PRO1 dyes simultaneously. This multicolor flow cytometry procedure requires 45 min for completion and provides a quantitative assessment of cell viability. It can be useful in evaluating the cytotoxic properties of new drugs, and antitumor interventions.
CITATION STYLE
Kudryavtsev, I., Serebryakova, M., Solovjeva, L., Svetlova, M., & Firsanov, D. (2017). Rapid detection of apoptosis in cultured mammalian cells. In Methods in Molecular Biology (Vol. 1644, pp. 105–111). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7187-9_8
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