Transcriptome‐facilitated development of SNPs for the Sonoran Desert rock fig, Ficus petiolaris (Moraceae)

Abstract

Premise of the study: Single-nucleotide polymorphism (SNP) primers were developed for a native North American desert Ficus pediolaris (Moraceae), to provide markers for population genetic studies designed to quantify patterns of gene How across a complex landscape. " Methods and Results: Transcriptome sequencing and bioinformatic protocols were implemented to discover SNPs in single-copy protein-coding genes. Multiplexes of 30 nuclear and 24 organellar (chloroplast and mitochondria]) SNPs were selected for primer development and genotyping on the Sequenom MASSArray System. Of these 54 loci, 49 reliably amplified across a panel of 96 petiolaris individuals. Conclusions: This study has provided SNP primers that can be applied in future studies investigating population genetics of F. petiolaris and its coevolution with associated pollinating and nonpollinating fig wasps.