Photoinduced electron transfer of chlorophyll in lipid bilayer system

Abstract

Photoinduced electron transfer from chlorophyll-a through the interface of dipalmitoylphosphatidylcholine (DPPC) headgroup of the lipid bilayers was studied with electron magnetic resonance (EMR). The photoproduced radicals were identified with electron spin resonance (ESR) and radical yields of chlorophyll-a Were determined by double integration ESR spectra. The formation of vesicles was identified by changes in measured λmax values from diethyl ether solutions to vesicles solutions indirectly, and observed directly with SEM and TEM images. The efficiency of photosynthesis in model system was determined by measuring the amount of chlorophyll-a radical yields which were obtained from integration of ESR spectra.