The "side population" (SP) phenotype is a manifestation of primitive cells' ability to efficiently efflux the fluorescent DNA-staining dye Hoechst 33342 and can be used as the basis by which to isolate these cells using flow cytometry. In the bone marrow (BM), the SP defines a cell subset with a highly homogeneous content of hematopoietic stem cells (HSCs). In this chapter, we describe a protocol to reproducibly isolate murine BM SP cells, as well as analytic measures, such as single cell transplantation, that can be used to assess the functionality of SP-derived stem cells. © Humana Press.
CITATION STYLE
Johnnidis, J. B., & Camargo, F. D. (2007). Isolation and functional characterization of side population stem cells. Methods in Molecular Biology, 430, 183–193. https://doi.org/10.1007/978-1-59745-182-6_13
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