The Toxicity of n-Hexane, Ethyl Acetate and Ethanol Extracts of SarangBanua (Clerodendrumfragrans Vent Willd) Leaves by Brine Shrimp Lethality Test (BSLT) Method

Abstract

Sarangbanua traditional medicinal plant is found in Simalungunand NorthTapanuli Regencies, Sumatera, Indonesia. The result of plant determination, sarangbanuais Clerodendrumfragrans Vent Willd, including the Verbenaceae family. This study aims to determine the toxicity of the three types of leaf extracts of C. fragrans Vent Will. The Brine Shrimp Lethality Test (BSLT) method was used to determine the toxicity of the extracts. The mortality data were then analyzed by Probit SAS to obtain LC50 values. The results showed that the three types of C. fragrans leaf extracts had LC50 values below 1000 µg/mL, so they were classified as toxic and potentially bioactive. The LC50 values of each of the ethanol, ethyl acetate and ethanol extracts were 26.25; 37.50 and 41.97 µg/mL