Endotoxin removal by end-line filters

Abstract

Four commonly used end-line filters, one with a charge-modified hydrophilic nylon filter (ELD96; Pall Biomedical Ltd., Portsmouth, United Kingdom), one with an unmodified nylon filter (FAE020; Pall Biomedical), and two with hydrophilic cellulose ester filters (Ivex-HP, Millipore Corp., Bedford, Mass.; Sterifix, Braun-Gelman, Brussels, Belgium), were evaluated for their endotoxin-removing capacity in saline and 5% glucose. Natural endotoxins derived from Escherichia coli 8739 and the lipopolysaccharide mutant Pseudomonas aeruginosa PA220-R2 and a purified E. coli serotype O111:B4 lipopolysaccharide preparation were used to challenge the four end-line filters. No endotoxin-removing capacity was observed for the Ivex-HP and Sterifix filters. Both the FAE020 and the ELD96 end-line filters showed excellent endotoxin-eliminating capacities in 5% glucose. Increasing the NaCl concentration in 5% glucose, however, greatly reduced the endotoxin-removing efficiency of especially the ELD96 filter for the purified E. coli lipopolysaccharide preparation. Obviously, both the nature of the endotoxin and the ionic strength of the solution had a major influence on the endotoxin end-line filtering efficiency.