Investigating the quantitative nature of MALDI-TOF MS

136Citations
Citations of this article
207Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

MALDI-TOF MS has been applied by several groups to relative quantitative measurements. At the same time, the non-quantitative character of this method has been widely reported. We conducted experiments to test the reliability of this technique for quantitation using the statistical method of inverse confidence limit calculation for the first time in this context. The relationship between relative intensities of known amounts of standard peptides and their concentration ratios was investigated. We found that the concentration ratios determined by relative intensity measurements were highly inaccurate and strongly influenced by the molecular milieu of the sample analyzed. Thus, we emphasize the necessity of using the sample itself for calibration. We also performed experiments using an isotope-labeled derivative of the analyte as an internal standard for calibration line generation. As expected, the use of such standard led to a dramatic increase in precision and a less pronounced improvement in accuracy. We recommend performing a similar statistical analysis as a demonstration of reliability for every system where MALDI-TOF MS is used for quantitative measurements. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

Cite

CITATION STYLE

APA

Szájli, E., Fehér, T., & Medzihradszky, K. F. (2008). Investigating the quantitative nature of MALDI-TOF MS. Molecular and Cellular Proteomics, 7(12), 2410–2418. https://doi.org/10.1074/mcp.M800108-MCP200

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free