The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.
CITATION STYLE
Otomo, K., Hibi, T., Murata, T., Watanabe, H., Kawakami, R., Nakayama, H., … Nemoto, T. (2015). Multi-point scanning two-photon excitation microscopy by utilizing a high-peak-power 1042-nm laser. Analytical Sciences, 31(4), 307–313. https://doi.org/10.2116/analsci.31.307
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