Protective effect of green tea extract against cytotoxicity induced by enrofloxacin in rat

Abstract

Enrofloxacin is bactericidal and has excellent activity against both Gram- positive and Gram-negative pathogens.120 male and female adult albino rats (Rattus norvegicus) were allotted among three groups. The animal were given daily 75mg/kg of enrofloxacin intraperitonealy followed by injection of green tea extract 1%,1.5% and 3% for ten days. Various Structural and numerical chromosomal aberrations in bone marrow cells; mitotic activity and sperm head abnormality were recorded, quantitated, and statistically analyzed. Also DNA extraction and apoptosis detection in liver, kidney, and spleen was done; in addition to RNA damage was detected in lysate tissues. The intensity of apoptotic bands located at 200 b p; 400b p ; 600 b p; 800 b p and intact DNA measured by software Gel Pro program as maximum optical density values. Enrofloxacin had adverse effect on chromosomal and sperm head structure, also it induce apoptosis, necrosis and decrease total RNA in rat tissues. Green tea extract attenuate the enrofloxacin-related toxic effects. The most potent dose of Green tea extract was1% and the less effective was 3%.