Magnesium-binding architectures in RNA crystal structures: Validation, binding preferences, classification and motif detection

Abstract

The ubiquitous presence of magnesium ions in RNA has long been recognized as a key factor governing RNA folding, and is crucial for many diverse functions of RNA molecules. In this work, Mg 2+ -binding architectures in RNA were systematically studied using a database of RNA crystal structures from the Protein Data Bank (PDB). Due to the abundance of poorly modeled or incorrectly identified Mg 2+ ions, the set of all sites was comprehensively validated and filtered to identify a benchmark dataset of 15 334 'reliable' RNA-bound Mg 2+ sites. The normalized frequencies by which specific RNA atoms coordinate Mg 2+ were derived for both the inner and outer coordination spheres. A hierarchical classification system of Mg 2+ sites in RNA structures was designed and applied to the benchmark dataset, yielding a set of 41 types of inner-sphere and 95 types of outer-sphere coordinating patterns. This classification system has also been applied to describe six previously reported Mg 2+ -binding motifs and detect them in new RNA structures. Investigation of the most populous site types resulted in the identification of seven novel Mg 2+ -binding motifs, and all RNA structures in the PDB were screened for the presence of these motifs.