Electron microscopy in situ hybridization. (EM-ISH) represents a powerful method that enables the localization of specific sequences of nucleic acids at high resolution. We provide here an overview of three different nonisotopic EM-ISH approaches that allow the visualization of nucleic acid sequences in cells. A comparison of various methods with respect to their sensitivity and the structural preservation of the sample is presented, with the aim of helping the reader to choose a convenient hybridization procedure. The postembedding EM-ISH protocol that currently represents the most widely used technique is described in detail, with a special emphasis on the organization of the cell nucleus.
CITATION STYLE
Cmarko, D., & Koberna, K. (2007). Electron microscopy in situ hybridization: Tracking of DNA and RNA sequences at high resolution. Methods in Molecular Biology, 369, 213–228. https://doi.org/10.1007/978-1-59745-294-6_11
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