Studying of genetic diversity in Satsuma (Citrus unshiu) mandarin utilizing microsatellite markers

Abstract

Genetic diversity of forty-four genotypes of Satsuma cultivars from Mazandaran was assessed using Simple Sequence Repeat (SSR) markers. SSR markers in different genotypes determined 2-5 alleles. The average of Polymorphic Information Content (PIC) in SSR markers were 0.88. Also, Principal Component Analysis (PCA) showed that SSR with four primary factors explained 80% of variations. Dendrograms showed that SSR markers could completely separate the Unshiu population. All the genotypes were grouped in 3 distinct clusters in cluster analysis. The control population, owari Genotypes were located in a separated cluster, while local Unshiu genotypes, unknown Unshiu, Wase and Wase derived genotypes were put together in another cluster. These results confirm the close relationship between local unshiu genotypes and Wase group or one of its similar varieties. © 2009 Academic Journals Inc.