Cloning and expression of an endo-1,3-1,4-β-glucanase Gene from Bacillus macerans in Lactobacillus reuteri

Abstract

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4β- glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bg/M gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.