Isolasi Bakteri Asam Laktat (BAL) dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) dan Kemampuannya Dalam Menghambat Staphylococcus aureus dan Shigella sp.

Abstract

Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.