Four variants of bifunctional HER2-specific recombinant proteins (anti-HER2-toxins) were created composed of HER2-specific scFv antibody (4D5scFv) or HER2-specific DARPin (D29) as targeting module and Pseudomonas exotoxin A fragment (PE40) as toxic module, and distinguished by the way of bacterial expression (cytoplasmic or periplasmic). Physicochemical, immuno- chemical, and functional properties of the created recombinant proteins as well as their expression yields were analyzed and compared with the option of the most promising one, D29-PE40, for further implementation as an agent for targeted therapy of HER2-overexpressing tumors. Keywords
CITATION STYLE
Deyev, S. M., Kutova, O. M., Lebedenko, E. N., Proshkina, G. M., Schulga, A. A., & Sokolova, E. A. (2018). Bifunctional Recombinant Protein Agent Based on Pseudomonas Exotoxin A Fragment for Targeted Therapy of HER2-Positive Tumors. In Proceedings of the Scientific-Practical Conference “Research and Development - 2016” (pp. 563–572). Springer International Publishing. https://doi.org/10.1007/978-3-319-62870-7_59
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