Isolation and identification of a bacterial strain with pullulanase activity and the cloning of the pullulanase Gene

Abstract

A novel bacterial strain OPF-0031 with pullulanase activity was isolated from mud sample of the sea. With the analysis of the 16S rDNA sequence and the characterization of biochemical reactions, the strain was identified to be Bacillus cereus. A 2641 bp DNA fragment including a pullulanase gene from the genome of OPF-0031, was present. The nucleotide sequence of the gene encoding pullulanase was cloned and analyzed in Escherichia coli. © Springer-Verlag Berlin Heidelberg 2014.