Ubiquitin cross-reactive protein is released by the bovine uterus in response to interferon during early pregnancy

Abstract

A 16-kDa protein has been identified that is secreted by the bovine endometrium in response to conceptus-derived interferon (IFN)-τ during early pregnancy. Because this uterine protein was similar in size to a human ubiquitin cross-reactive protein (hUCRP) that also was regulated by IFN, we suspected that they might be related. To test this hypothesis, uterine flushings, medium from cultured endometrium, and endometrial tissues were examined for the presence of ubiquitin-immunoreactive proteins. Immunoreacting proteins were detected through use of one-dimensional (1D)- PAGE and Western blotting with ubiquitin and hUCRP antiserum (1:500). A 16- kDa protein that cross-reacted with ubiquitin and hUCRP antisera was released by the endometrium and was present in uterine flushings from all Day 18 pregnant females examined (n = 12). The immunoreacting 16-kDa protein was absent in all nonpregnant females examined (n = 23). Regulation of this uterine protein by recombinant type I IFNs (rbIFN-τ, rbIFN-α, and roIFN- τ), using 0, 0.5, 5, and 25 nm of each IFN, was evaluated in nonpregnant (Day 12) heifers (n = 5) using 1D-PAGE and Western blotting. Release of the 16-kDa protein into medium was negligible in controls (0 nm IFN). For each IFN, a dose-dependent increase (p < 0.05) in release of the immunoreacting 16-kDa protein was noted. We conclude that the 16-kDa protein that is produced by the endometrium in response to IFN-τ during early pregnancy also shares epitopes with hUCRP and ubiquitin. The 16-kDa protein has been named bovine UCRP.