Molecular chemotyping of Fusarium graminearum, F. culmorum, and F. cerealis isolates from Finland and Russia

Abstract

PCR assays that yield markers that are predictive of NIV versus DON production or 3ADON versus 15ADON production were performed for 60 Fusarium graminearum isolates, most of which were from Finland and Russia. None of the F. graminearum isolates originating from Finland and north-western Russia produced any PCR fragment with DON and NIV specific primers, which indicates that they have a 3ADON chemotype. All F. graminearum isolates from southern Russia and most of the isolates from Asia and Germany produced fragments typical of the 15ADON chemotype. All F. culmorum isolates belonged to 3ADON chemotype, while all F. cerealis isolates belonged to NIV chemotype. The highest DON levels were found in the 3ADON molecular chemotype isolates of Finland, north-western Russia, and central Russia. In the combined 3ADON molecular chemotype isolates, DON production was clearly higher than in the combined 15DON chemotype isolates. © 2010 Springer-Verlag Berlin Heidelberg.