Abstract
Differential phosphorylation of proteins is a key regulatory mechanism in biology. Immunoprecipitation-coupled mass spectrometry facilitates the targeted analysis of transient receptor ion potential channel polycystin-2 (TRPP2) phosphorylation. However, empirical testing is required to optimize experimental conditions for immunoprecipitation and mass spectrometry. Here, we present a detailed workflow for the reliable analysis of endogenous TRPP2 phosphorylation in differentiated renal epithelial cells.
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Dumit, V. I., Köttgen, M., & Hofherr, A. (2019). Mass spectrometry-based analysis of TRPP2 phosphorylation. In Methods in Molecular Biology (Vol. 1987, pp. 51–64). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9446-5_4
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