Rat model of healing the skin wounds and joint inflammations by recombinant human angiogenin, erythropoietin and tumor necrosis factor-α

Abstract

This study is a part of an effort on the development of a new generation of wound healing medicines based on recombinant human peptides and cytokines, namely angiogenin (rhu-ANG), erythropoietin (rhu-EPO) and tumor necrosis factor alpha (rhu-TNF-α). The screening of a few drug matrixes was conducted and high purity polyethylene oxide was found to be the most appropriate one. Three gel forms of peptides were concocted and investigated in the study. Four rat models of surgical skin muscle wounds and two of joint acute and adjuvant arthritic inflammations were developed and tested with foresaid gels in comparison with conventional gel Solcoseryl and Butadion ointment. Plethora of morpho-cytological and hematological data was produced for all tested materials and improvement of wound/inflammation healing process with all peptides compared to Solcoseryl and Butadion was proven. © 2011 Springer-Verlag.