Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping.
CITATION STYLE
Steffensen, R., Baech, J., & Nielsen, K. R. (2015). Allelic discrimination by TaqMan-PCR for genotyping of human neutrophil antigens. Methods in Molecular Biology, 1310, 205–212. https://doi.org/10.1007/978-1-4939-2690-9_17
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