mRNA detection by whole mount in situ hybridization (WISH) or sectioned tissue in situ hybridization (SISH) in Arabidopsis.

Abstract

Gene expression can be analyzed at high spatial resolution via RNA in situ detection methods. For many tissues and species, these will be performed on sections of embedded and fixed plant material. When very small or fragile tissues, such as embryos or roots are being investigated, whole mount methods can be employed. Protocols for both approaches are described in detail.