Screening of novel subunits of chloroplastic NAD(P)H dehydrogenase in Arabidopsis

Abstract

Chloroplastic NAD(P)H dehydrogenase is a homolog of respiratory complex I and involved in cyclic electron flow around photosystem I. Recent studies have revealed the difference in subunit constitution between chloroplastic and respiratory complex. Our in silico screening of subunits in chloroplastic NDH based on co-expression analysis identified several novel subunits including NDF6 which has a trans-membrane domain and would be specific to terrestrial plants. We discuss the efficiency of combining screening methods of novel subunits in chloroplastic NDH using the co-expression and phylogenic analysis.