RNA turnover is an essential part of the gene expression pathway, and there are several experimental approaches for its determination. High-throughput measurement of global RNA turnover rates can provide valuable information about conditions or proteins that impact gene expression. Here, we present a protocol for mitochondrial RNA turnover analysis which involves metabolic labeling of RNA coupled with quantitative high-throughput fluorescent microscopy. This approach gives an excellent opportunity to discover new factors involved in mitochondrial gene regulation when combined with loss-of-function screening strategy.
CITATION STYLE
Kotrys, A. V., Borowski, L. S., & Szczesny, R. J. (2021). High-Throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells. In Methods in Molecular Biology (Vol. 2192, pp. 133–146). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0834-0_11
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