Production of poly hydroxy butyrate (PHB) from Eichhornia crassipes through microbial fermentation process

Abstract

Polyhydroxybutyrate (PHB) is one of the highly biodegradable and biologically acceptable thermoplastics synthesized by many microorganisms collectively called polyhydroxyalkanoates (PHAs). All available biopolymers are viewed as perfect answers for the resolution of natural contamination issue by supplanting ordinary plastic business. They are likewise utilized as osteosclerotic stimulants attributable to their piezoelectric properties, in bone plates, during operations as suture material and vein substitutions. Synthesis of PHB is found in a wide range of Gram’s negative and gram’s positive bacteria belonging to distinct genera. Optimum culture condition for the PHB producing microbes are provided, including restricted centralization of nitrogen, sulfur, phosphorus, or the trace elements and maximum convergence of carbon source Indeed, to market PHAs, significant exertion has been dedicated towards a decline in the production cost through the improvement of bacterial strains and enhancing effectiveness of recovery/fermentation procedure. This is being done considering the fact that substrate prices show the greatest impact on PHA's manufacturing cost. The price of the substrate used has the most significant influence on the production cost of PHA. In this research, a potential bacterial strain was isolated from the soil and tested for its PHB producing ability. The use of cheaper substrate for lowering the cost is prerequisite. For PHB production, water hyacinth was used as a carbon source. Bacterial growth was optimized for maximum PHB production. The optimum condition was found to be 30 °C, 8% substrate concentration and 72 h of incubation time.