Antioxidant capacity profile of dewandaru leaf (extract eugenia uniflora l.): part of usadha Bali

Abstract

Bali has around 50,000 usadha palm oil which is a source of untreated herbal medicine. One of the plants that have the potential as an antioxidant and is traditionally used by the community is Dewandaru (Eugenia uniflora L.) plant. Dewandaru containing tannins, flavonoids, and anthocyanins. Dewandaru plants have antibacterial activity, antioxidants, and antidotes to free radicals.  Making extract with six different solvents, among them methanol, ethyl acetate, n-butanol, chloroform, and n-hexane. The measurements were taken of the reduction of free radical activity by observing the absorbance at the maximum wavelength with a UV-Vis spectrophotometer alternately on the six samples. Secondary metabolites contained in Dewandaru leaves, namely flavonoids, tannins, and quinones. The value obtained is IC50 methanol extract 5,857 ppm; n-butanol extract 8,893 ppm; ethyl acetate extract 15,203 ppm; n-hexane extract 162.7315 ppm; and chloroform extract 75,873 ppm. Methanol extract, n-butanol, and ethyl acetate have very strong antioxidant activity because <50 ppm; n-hexane extract has weak antioxidant activity (ranging from 150-200 ppm), and chloroform extract has strong antioxidant activity (ranging from 50-100 ppm). Major conclusions: Dewandaru leaves has the potential as a natural antioxidant in the treatment section of Usadha Bali.