In vivo lentiviral gene delivery of hla-dr and vaccination of humanized mice for improving the human t and b cell immune reconstitution

5Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

Humanized mouse models generated with human hematopoietic stem cells (HSCs) and reconstituting the human immune system (HIS-mice) are invigorating preclinical testing of vaccines and immunotherapies. We have recently shown that human engineered dendritic cells boosted bonafide human T and B cell maturation and antigen-specific responses in HIS-mice. Here, we evaluated a cell-free system based on in vivo co-delivery of lentiviral vectors (LVs) for expression of a human leukocyte antigen (HLA-DRA*01/ HLA-DRB1*0401 functional complex, “DR4”), and a LV vaccine expressing human cytokines (GM-CSF and IFN-α) and a human cytomegalovirus gB antigen (HCMV-gB). Humanized NOD/Rag1null/IL2Rγnull (NRG) mice injected by i.v. with LV-DR4/fLuc showed long-lasting (up to 20 weeks) vector distribution and expression in the spleen and liver. In vivo administration of the LV vaccine after LV-DR4/fLuc delivery boosted the cellularity of lymph nodes, promoted maturation of terminal effector CD4+ T cells, and promoted significantly higher development of IgG+ and IgA+ B cells. This modular lentigenic system opens several perspectives for basic human immunology research and preclinical utilization of LVs to deliver HLAs into HIS-mice.

Cite

CITATION STYLE

APA

Kumar, S., Koenig, J., Schneider, A., Wermeling, F., Boddul, S., Theobald, S. J., … Stripecke, R. (2021). In vivo lentiviral gene delivery of hla-dr and vaccination of humanized mice for improving the human t and b cell immune reconstitution. Biomedicines, 9(8). https://doi.org/10.3390/biomedicines9080961

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free