Minimum origin requirements for linear duplex AAV DNA replication in vitro

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Abstract

We have investigated the minimal requirements for a functional origin of DNA replication in an in vitro assay which requires the adeno-associated virus (AAV) Rep 68/78 protein. When a linear duplex template was used, initiation of one round of DNA replication was achieved when nucleotides 1 ˜56 of the AAV inverted terminal repeat (in the flop orientation) were present at the terminus of the template. A terminal resolution site was not required. Deletion of an additional 11 nucleotides from the terminal repeat blocked the reaction. © 1995 Academic Press, Inc.

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Ward, P., & Berns, K. I. (1995). Minimum origin requirements for linear duplex AAV DNA replication in vitro. Virology, 209(2), 692–695. https://doi.org/10.1006/viro.1995.1306

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