Protein N-terminal acetylation is a widespread modification in eukaryotes catalyzed by N-terminal acetyltransferases (NATs). The various NATs and their specific substrate specificities and catalytic mechanisms are far from fully understood. We here describe an in vitro method based on reverse-phase HPLC to quantitatively measure in vitro acetylation of NAT oligopeptide substrates, enabling the determination of NAT specificity as well as kinetic parameters. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Evjenth, R. H., Van Damme, P., Gevaert, K., & Arnesen, T. (2013). HPLC-based quantification of in vitro N-terminal acetylation. Methods in Molecular Biology, 981, 95–102. https://doi.org/10.1007/978-1-62703-305-3_7
Mendeley helps you to discover research relevant for your work.