Genetic effect of the aegilopscaudataplasmon on the manifestation of the ae.Cylindricagenome

Abstract

In the course of reconstructing Aegilopscaudatafrom its own genome (CC) and its plasmon, which had passed half a century in common wheat (genome AABBDD), we produced alloplasmicAe. cylindrica(genome CCDD) with the plasmon of Ae. caudata. This line, designated (caudata)-CCDD, was found to express male sterility in its second substitution backcross generation (SB2) of (caudata)-AABBCCDD pollinated three times with the Ae. cylindricapollen. We repeatedly backcrossed these SB2 plants with the Ae. cylindricapollen until the SB5 generation, and SB5F2 progeny were produced by self-pollination of the SB5 plants. Thirteen morphological and physiological characters, including pollen and seed fertilities, of the (caudata)-CCDD SB5F2 were compared with those of the euplasmicAe. cylindrica. The results indicated that the male sterility expressed by (caudata)-CCDD was due to genetic incompatibility between the Ae. cylindricagenome and Ae. caudataplasmon that did not affect any other characters of Ae. cylindrica. Also, we report that the genome integrity functions in keeping the univalent transmission rate high.