Assessment of secondary metabolites in in vitro regenerated plantlets of oroxylum indicum (L.) vent

Abstract

Flavonoids baicalein and baicalein-7-o-glucoside were estimated in in vitro raised (six months old) as well as nature grown (12 months old) plants of Oroxylum indicum (L.) Vent. Multiple shoot formation and elongation were obtained from axillary and apical bud expiants of O. indicum on MS containing BAP (1 mg/1) and AgNQ3 (2 mg/1). Rooting of the regenerated shoots was achieved on medium (half strength) containing IBA (1 mg/1) and AgNO 3 (1 mg/1). A quantitative HPLC analysis of methanolic extract of leaf and root indicated the enhancement of baicalein-7-o glucoside in in vitro raised root and leaf of Oroxylum indicum.