Signaling role of CD36 in platelet activation and thrombus formation on immobilized thrombospondin or oxidized low-density lipoprotein

Abstract

Background and Objective:Platelets abundantly express glycoprotein CD36 with thrombospondin-1 (TSP1) and oxidized low-density lipoprotein (oxLDL) as proposed ligands. How these agents promote platelet activation is still poorly understood. Methods and Results:Both TSP1 and oxLDL caused limited activation of platelets in suspension. However, immobilized TSP1 and oxLDL, but not LDL, strongly supported platelet adhesion and spreading with a major role of CD36. Platelet spreading was accompanied by potent Ca 2+ rises, and resulted in exposure of P-selectin and integrin activation, all in a CD36-dependent manner with additional contributions of α IIbβ 3 and ADP receptor stimulation. Signaling responses via CD36 involved activation of the protein tyrosine kinase Syk. In whole blood perfusion, co-coating of TSP1 or oxLDL with collagen enhanced thrombus formation at high-shear flow conditions, with increased expression on platelets of activated α IIbβ 3, P-selectin and phosphatidylserine, again in a CD36-dependent way. Conclusions:Immobilized TSP1 and oxLDL activate platelets partly via CD36 through a Syk kinase-dependent Ca 2+ signaling mechanism, which enhances collagen-dependent thrombus formation under flow. These findings provide novel insight into the role of CD36 in hemostasis. © 2011 International Society on Thrombosis and Haemostasis.