ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL OF Caulerpa sertularoides AND Padina australis FROM NAIN ISLAND, NORTH SULAWESI, INDONESIA

Abstract

Methanol extract of Caulerpa sertularoidesand Padina australiswere screened against Gram positive (Staphylococcus aureusATCC 6538) and Gram negative (Escherichia coliTCC 25922) bacteria. The methanol extract were further extract by multiple phase partitioning method using water, ethyl acetate and hexane and also screened against those bacteria. The hexane fraction of P.australis showed high antibacterial activity against S.aureus(inhibitory zone diameter > 20 mm). Phytochemistry result showed that ethyl acetate fraction from C. sertularoidesextract has phenolic, flavonoid, steroid, triterpenoid, saponin and tanin. Overall, it can be concluded that the methanol and its fractions extract from both types of marine algae have antibacterial potency against