The ability to prepare accurate solutions, reagents, and buffers will determine the outcome of a successful experiment. Much time and effort spent in a lab will consist of making solutions, reagents, and buffers. This chapter introduces and refreshes the skill of making solutions including: molarity (M) concentration, percentage (%) concentration, mass/volume concentrations, X-factor concentration, working concentrations from stock solutions, conversions from percentage (%) to molarity (M) concentration, and conversions from molarity (M) to percentage (%) concentration. Basic formulas and examples for how to make solution concentrations are covered.
CITATION STYLE
Singleton, T. L. (2018). Preparation of solutions and reagents. In Methods in Molecular Biology (Vol. 1721, pp. 1–7). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7546-4_1
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