Isolation and characterization of anaerobic bacteria from frozen rumen liquid and its potential characterizations

Abstract

Background and Objective: Anaerobic bacteriadiversity in the rumen could be one of the keys of enhancing ruminant performance and productivity. The anaerobic population by its great diversity could be possible solution for some nutritional properties and enhance animal resistance to pathogenic microbes. Antimicrobial resistance for antibiotics used in animal production. The main effects of probiotics are the improved resistance to pathogenic bacteria colonization and enhanced host mucosa immunity; thus resulting in a reduced pathogen load, an improved health status of the animals and a reduced risk of food-borne pathogens in foods. This study aimed to isolate anaerobic probiotic from bovine rumen samples. Materials and Methods: Isolation were carried out using classical method by using selective medium then the isolates were identified using modern techniques by real time PCR and DNA sequencing techniques. Results: Results showed identified isolates were as Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum. These isolates showed a great cellulytic activity by producing cellulase enzyme with activity degree as 6543, 8555 and 5179 IU g-1 for Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum, respectively. Moreover, the activity of isolates for degrading tannins were 598, 1402 and 866 U g-1 for Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum, respectively. Clostridium butyricum showed superiority for inhibiting pathogenic bacteria (Yersinia enterocolitica, Bacillus cereus and Staphylococcus aureus) while, Blautia obeum recorded the highest antimicrobial activity against Salmonella typhimurium. Ruminococcus flavefaciens showed the higher activity against Listeria monocytogenes, Klebsiella pneumonia and Clostridium perfringens Conclusions: The results showed a potential properties of Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum to be used in as supplement to ruminant diet to enhance its performance and improve the health status through the antimicrobial activity of the isolated bacteria (Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum).