Control of leukocyte rolling velocity in TNF-α-induced inflammation by LFA-1 and Mac-1

Abstract

Previously it was shown that β2-integrins are necessary for slow leukocyte rolling in inflamed venules. In this study, mice that are deficient for either one of the β2-integrins, αLβ2 (LFA-1) or αMβ2 (Mac-1), were used to determine which of the β2-integrins are responsible for slowing rolling leukocytes. The cremaster muscles of these mice were treated with tumor necrosis factor-α and prepared for intravital microscopy. The average rolling velocities in venules were elevated in LFA-1-/- mice (11.0 ± 0.7 μm/s) and Mac-1-/- mice (10.1 ± 1.1 μm/s) compared to wild-type mice (4.8 ± 0.3 μm/s; P