Large-scale purification of IgM from human sera - Comparison of Three Optimized Procedures Utilizing Protein A Chromatography

Abstract

For the preparation of gram amounts of IgM from human sera sedimention at 100,000 g or treatment with ZnSO4 of the redissolved "euglobulin"-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably. © 1980 Springer-Verlag.