Evaluation of antibacterial and antioxidant activities of sacha inchi (Plukenetia volubilis L.) leaf from Myanmar

Abstract

The comparative study evaluated the antioxidant and antimicrobial activities of Sacha inchi (Plukenetia volubilis L.) leaf extracts from Mandalay and Yangon, Myanmar. The total polyphenol and flavonoid contents were higher in Sacha inchi extracts from Yangon (97.84 and 89.60 mg/g, respectively) than in those from Mandalay (71.27 and 60.81 mg/g, respectively). Both DPPH and ABTS radical scavenging activities and FRAP values were higher in Sacha inchi from Yangon, than Mandalay. A clear zone formed aroundallpaperdiscs placed on a plate coated with pathogenic bacteria indicated antibacterial activity of Sacha inchi extracts. Time-kill curves showed that the growth of all tested strains was inhibited by incubation with Sacha inchi extracts for 24 h. The MIC50 and MIC100 were 6.25-2,000 and 125-5,000 µg/mL for eleven and eight tested strains. All measurement methods showed that the antibacterial activity was superior for Sacha inchi from Yangon compared with Mandalay. The more effective antioxidant and antimicrobial activities of Sacha inchi from Yangon were ascribed to the higher abundance of functional phenolics and flavonoids.