endo-1,4-β-Glucanase (endo-cellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In 2014, a novel enzyme-coupled assay that addressed many of the limitations of the existing assay methodology was reported. This involved the use of a bifunctional substrate chemically derived from cellotriose. Reported herein is a much improved version of this assay employing a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-d-cellopentaoside. [Figure not available: see fulltext.]
CITATION STYLE
Mangan, D., Cornaggia, C., McKie, V., Kargelis, T., & McCleary, B. V. (2016). A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase). Analytical and Bioanalytical Chemistry, 408(15), 4159–4168. https://doi.org/10.1007/s00216-016-9507-y
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