The hexahistidine containing organophosphorus hydrolase enzyme and bacterial cellulose based functional materials

Abstract

Bacterial cellulose (BC) is a unique biopolymer for creation on its basis functional materials used as drugs, matrices for regenerative therapy, dressings, etc. In this study, the possibility of combining of BC with hexahistidine containing organophosphorus hydrolase (His6-OPH), exhibiting lactonase activity against gram-negative pathogenic bacteria is considered to obtain composite materials with special properties. The dry and wet samples of fibrous BC, impregnated with solutions of the His6-OPH enzyme and various antimicrobial agents were obtained; their catalytic characteristics were investigated. The preservation of the enzymatic activity of the His6-OPH enzyme in the composition of the obtained samples with BC, including after the lyophilization process, has been established. The stabilization of the enzymatic activity of His6-OPH in the composition of fibrous materials in the presence of poly(aspartic) acid and antimicrobial agents is shown.