Manufacturing chimeric antigen receptor (CAR)-modified T cells requires incorporation of the CAR transgene, for which viral vectors are most often used. Here, we describe the generation of CAR T cells using primary human T cells and a non–self-inactivating gammaretroviral vector encoding a CAR transgene. The gammaretroviral vector is produced by 293T cells transiently transfected with DNA plasmids encoding necessary components of the viral vector. The resulting viral particles efficiently infect activated T cells and integrate the CAR transgene into the genome of dividing cells for stable expression.
CITATION STYLE
Mo, F., & Mamonkin, M. (2020). Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors. In Methods in Molecular Biology (Vol. 2086, pp. 119–130). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0146-4_8
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