Preparing an injectable hydrogel with sodium alginate and Type i collagen to create better MSCs growth microenvironment

Abstract

In the past few decades, stem cell transplantation has been generally accepted as an effective method on the treatment of tissue and organ injury. However, the insufficient number of transplanted stem cells and low survival rate that caused by series of negative conditions limit the therapeutic effect. In this contribution, we developed an injectable hydrogel composed of sodium alginate (SA) and Type I collagen (ColI), as the tissue scaffold to create better growth microenvironment for the stem cells. Compared the traditional SA scaffold, the ColI/SA hydrogel inherits its biomimetic properties, and simultaneously has shorter gelation time which means less loss of the transplanted stem cells. The mesenchyma stem cell (MSC) culture experiments indicated that the ColI/SA hydrogel could prevent the MSC apoptosis and contributed to faster MSC proliferation. It is highlighted that this ColI/SA hydrogel may have potential application for tissue regeneration and organ repair as the stem cell scaffold.