Profiling genome-wide histone modifications and variants by ChIP-chip on tiling microarrays in S. cerevisiae

Abstract

Chromatin immunoprecipitation coupled to DNA microarray has become a widely used method to study transcription factors and chromatin structure. Here, we provide a detailed protocol for the localization of the variant histone Htz1 in the S. cerevisiae genome. This protocol can easily be adapted to fit other purposes such as profiling histone modifications. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.