Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity

Abstract

Zinc ions, methylated poly(1-vinylimidazole) (PVIm-Me) and plasmid DNA (pDNA) have formed ternary complexes for gene delivery. The resulting Zn-PVIm-Me-pDNA complexes have delivered both Zn 2+ ions and pDNA inside cells, leading to the nuclear translocation of the pDNA. By use of the pDNA containing a nuclear protein, NF-κB, binding sequence, the intracellular co-delivery of Zn 2+ ions and pDNA has enhanced gene expression. These results suggest that the intracellular Zn 2+ ions delivered by Zn-PVIm-Me-pDNA complexes activated the NF-κB, enhancing the nuclear translocation of the pDNA. In conclusion, it has been demonstrated that the Zn-PVIm-Me-pDNA complex is capable of enhancing the gene transfection activity by a synergic effect of the PVIm-Me and the co-delivered intracellular Zn 2+ ions. © 2014 The Royal Society of Chemistry.